POSTDOCTORAL FELLOW IN SPATIAL TRANSCRIPTOMICS - BOSTON CHILDREN'S

About the role

We are recruiting a postdoctoral fellow for the Moffitt laboratory at Harvard Medical School and Boston Children’s Hospital to leverage multiplexed-error robust fluorescence in situ hybridization (MERFISH) to reveal new insights into the cellular and molecular structure of human nociception and chronic pain.

Responsibilities

• Apply MERFISH and other spatial transcriptomics technologies—Visium and Stereo-Seq—to discover, define, and chart the cell types and states associated with human nociception and their disorders in the context of chronic phantom limb pain associated neuromas.
• Derive novel biological insights from these data on the structure, origin, and physiology of nociception in human neuromas.
• Learn and apply both image-based and spatial-capture-based transcriptomic methods to human-derived neuroma samples.
• Co-embed these data with data derived from other state-of-the-art single-cell methods, such as scRNA-seq and sc-ATAC-seq.
• Create novel experimental and computational extensions of MERFISH.
• Contribute to a dynamic team of scientists that are both extending this technology in novel and exciting ways and leveraging it to reveal new biological insights in a wide array of systems.

Requirements

• A PhD in the natural sciences, including but not limited to Molecular and Cell Biology, Neurobiology, Chemistry, Physics, or Bioengineering
• Experience with programming languages such as Matlab, Python, or R
• The ability to work well within teams
• The willingness to learn and develop new technologies, learn new areas of biology, and contribute to a deeper understanding of human neurological disorders and disease

Beneficial skills (not required)

• Experience with fluorescence in situ hybridization (FISH), including techniques such as single-molecule FISH, RNAscope, or MERFISH
• Microscopy and optics experience
• High-performance computing experience and a familiarity with Linux
• Experience with tissue preservation and sectioning, including sectioning of both fresh-frozen samples and paraffin-embedded, formalin-fixed (FFPE) samples
• Single-cell analysis experience, including software packages such as scanpy or Seurat
• Experience in neurobiology or neuroscience

Pay

TBD

Schedule

TBD

Benefits

TBD

Contact

To apply, please send a CV and the name of two references to jeffrey.moffitt@childrens.harvard.edu.

To learn more about the Moffitt laboratory visit: https://moffittlab.github.io.

To learn more about the Harvard PRECISION Pain Center visit: https://healpain.bwh.harvard.edu/.