Microbiology Technician- Pediatrics - 140440

About the role

The Department of Pediatrics at UC San Diego School of Medicine is seeking a Microbiology Technician to support innovative research aimed at developing treatments for antibiotic-resistant bacterial infections. This role collaborates with junior faculty, postdoctoral fellows, and graduate students, focusing on enhancing the immune system's response to pathogens.

Responsibilities

- Assists in the evaluation of novel anti-infective therapies that stimulate immune cell function. - Conducts experiments involving human tissue culture and mouse models of bacterial infection and anti-infective therapy. - Performs molecular genetics, microbiology, cell biology, and immunological analyses. - Manages laboratory tasks such as ordering supplies, maintaining databases, and overseeing student lab assistants. - Assists in preparing detailed and accurate scientific reports.

Requirements

- Theoretical knowledge of biochemistry and/or microbiology. - Experience in preparing scientific reagents. - Knowledge and experience with laboratory equipment, including centrifuges, bioguard hoods, microscopes, pipeters, autoclaves, balances, incubators. - General laboratory skills and safety training. - Ability to evaluate and communicate scientific data. - Ability to maintain data accurately and perform mathematical computations. - Basic computer skills including word processing and graphics applications. - Knowledge of sterile techniques for bacterial and cell culture. - Understanding of DNA, RNA, and protein analysis, PCR, and ELISA. - Ability to prepare detailed and accurate scientific reports. - Knowledge/experience to establish and work with lung organoids. - Knowledge of Murine model of infection (Pneumonia, bacteremia, skin and UTI). - Experience with monoclonal antibody development pipeline. - Knowledge of immunological profiling using flow cytometry. - Experience with protein purification and HPLC analysis.

Priorities

- Experience with mouse handling and experimentation. - Experience with tissue culture. - Occasional evenings and weekends may be required. - Must be willing to work with hazardous materials.

Special Conditions

- Employment is subject to a criminal background check. - Occasional evenings and weekends may be required. - Must be willing to work with hazardous materials.