Intermediate Level Research Services Professional

Key Responsibilities / Duties

• Animal work (20%)
• Tagging and genotyping of mice
• Auxiliary with metabolic studies in mice
• Dissect and process mouse tissues for immunohistochemical analysis
• Routine and advanced molecular biology, biochemistry, bioinformatics, and cell biology techniques (60%)
• Standard bench work (e.g., ELISA, PCR and RT-PCR, DNA and RNA purification, protein extraction and western blotting, tissue culture, immunofluorescent staining and confocal microscopy)
• Culture cell lines and primary tissue (islets)
• Differentiate human embryonic stem cells to islet cells
• Optimize and establish advanced molecular biology techniques including analysis of gene expression (single cell sequencing and ribosome profiling), RNA decay, and mRNA translation
• Bioinformatics analysis of available single-cell and proteomics datasets
• General lab duties (20%)
• Take inventory of laboratory supplies and order new supplies as necessary
• Equipment setup, maintenance, and repair
• Manage chemical inventories and arrange pickup of chemical waste
• Act as laboratory safety coordinator
• Assist and train junior team members

Qualifications

• Minimum Qualifications: Bachelor’s degree in biology, biochemistry, physiology, molecular biology, genetics or related field
• One (1) year of experience in an experimental or wet laboratory

Conditions of Employment

• Must be willing and able to work with live mice
• Must be in commuting distance, or willing to relocate to commuting distance, to Aurora, CO