Core Technologist Sr

Target hiring range for this position is between $25.05-$31.31 per hour. Offered salary will be determined by the applicant’s education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

About the role

Provides daily work oversight and executes research activities. Oversees service workflow in consultation and support of users and junior staff executing procedures. Assists in the development and implementation of new core services, and other core laboratory and research functions. Ensures overall quality of research functions and responsible for unit's compliance in regard to University, sponsor and federal rules and regulations.

Responsibilities

Administration

• Ensures client billing information is complete and accurate.
• Compiles billing information for documenting usage and billing information into the Core's automated service tracking/billing system.
• Develops cost analyses on services rendered by the Core.
• Assists in preparing cost quotes on experimental design requested by investigators.
• Ensures that all work is accurately and completely documented.

Collaboration

• Interacts with both Core Staff and clients in the planning and execution of experiments/core services.
• Advises PIs as appropriate.

Compliance

• Responsible for the Core's compliance with safety protocols, relative to the use of combustibles, radioactivity, toxic material, and/or carcinogenics.
• Ensures sterile conditions as required.
• Prepares reagents.
• Ensures compliance with all University, sponsor and federal rules and regulations.

Scientific

• Plans, conducts and evaluates experiments and assays of varying complexity.
• Understands, follows, and troubleshoots protocols.
• Records, compiles, and reports data.
• Provides analysis and insightful interpretations of results.
• Participates in beta-testing of new protocols and services.

Technical

• Develops new methods or customizes protocols for users.
• Ensures lab is prepared for service delivery (experimentation).
• Ensures established inventory levels, and notes usage trends so that inventory levels meet client demand.
• Ensures the recommended maintenance on instrumentation and equipment is performed according to manufacturer and University guidelines.
• Troubleshoots equipment problems, and mitigates the issues with training and/or repair protocols.
• Recommends equipment improvements and/or new equipment.
• Assists in the development of usage protocols for new equipment.

Using an advanced level of skill, completes the following activities:

Molecular Biology

• DNA, RNA, Protein isolation, purification and expression
• Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Recombinant virus production and purification

Cellular Biology

• Cell culture, cell isolation
• Functional studies
• Transfection
• Immunofluorescence assays
• Image analysis and quantification

Chemistry

• Analyze organic chemistry
• Column chromatography
• Chromatography
• Enzymatic digestions
• HPLC
• NMR
• Mass spectrometry
• Protein extractions
• Solid phase extraction

Histology

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry

Microscopy

• Immunology
• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

Animal Husbandry

• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony

Equipment Used

• Analytical balance
• Autoclave
• Blood gas analyzers
• Centrifuge
• Electrophoresis unit
• pH meter
• UV/Vis Spectrophotometer
• Scintillation counter
• Plate reader
• Sequencers
• Real-time qPCR and digital PCR instruments
• Other genomics instruments

Training

• Trains or assists in training of technicians and trainees including students.
• Provides training to researchers in instrument and equipment operation.

Miscellaneous

• Perform other duties as assigned.

Requirements

Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree; 4 years of technical or other relevant experience.

Benefits

• Health, dental, vision, disability, and life insurance
• Paid vacation and holidays
• Paid medical/sick and parental leave
• Tuition benefits for the employee and dependents
• Pre-tax and flex spending accounts for commuting and dependent care
• Retirement savings options
• Wellness programs
• Work-life and wellness programs including flexible work arrangements, childcare, and senior/adult care support
• Professional growth and development resources