Core Rsch Facil Technician III - Proteomics

Job Description

Process examples according to SOP for analysis on the timsTOF-MS and Orbitrap Fusion MS; contribute to new method development on this instrument.

Review independent protein quality control (QC) data and assist routine customer projects involving in-gel and in-solution protein digestion, precipitated protein and tissue processing, protein identification, and label-free proteomics by mass spectrometry using the LC-MS/MS (timsTOF fleX, Orbitrap Fusion, and TSQ Altis) instrumentation.

Assist the Scientific Director in consulting with customers to coordinate sample collection and project deliverables, and in communicating analytical results.

Perform independent data analysis via database searches on raw data files generated.

Ensure project deadlines are met. Communicate with customers through iLab or email to provide project updates and address any additional needs via iLab.

Complete the standard protocols and record all completed work for billing in iLab on a weekly basis.

Perform sample accessioning in accordance with ICBR and PM policies. Prepare Statement of Work (SOW) prior to accepting and processing customer samples, and ensure proper approval from the customer.

Generate a final data delivery document compared to SOW.

Assist in maintaining laboratory equipment for daily maintenance procedures, including quality control, checking solvents, and gases to ensure they do not run out.

Learn and execute backup duties for other protocols and equipment.

Qualifications

• BA/BS in a related scientific field, or an equivalent combination of education and experience.
• BS/BA in Biochemistry, Biotechnology, Analytical Chemistry, or related field preferred.
• Previous experience with protein sample preparation and fractionation, with a basic understanding of liquid chromatography.
• Experience in Mass spectrometry and proteomics data analysis (Mascot, Scaffold, ProteinPilot, Proteome Discoverer, Compound Discoverer, MaxQuant) and acquisition (Xcalibur, timsControl, OtoControl, Hystar).