Biochemist I/II - Recombinant Protein Manufacturing

Job Summary

This position is responsible for manufacturing cutting edge recombinant protein research products in Molecular Cellular Biology (MCBio) group at BioLegend. The Recombinant Manufacturing Biochemist will be involved in different stages of production of various recombinant protein products, which includes recombinant protein expression and purification.

Responsibilities

• Prepare various buffers for experiments, including cell culture media.
• Perform transformation and culture of bacterial cells (1-50 liters).
• Process the culture supernatant/cell pellet/inclusion body, followed by extensive processing of the supernatant/cell pellet/inclusion body or diafiltration to extract or refold raw protein bulk.
• Support purifying recombinant protein by means of affinity tag.
• Support technical training for MFG procedures.
• Support activities related to audit readiness, CAPA and NC investigation.
• Monitor processing by SDS-PAGE for varied protein production stages.
• Perform bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal.
• Maintain inventory and record for recombinant protein products.
• Affirm inventory management of raw material and plasmid constructs for protein production.
• Assist in transferring products across different departments for QC or other inventory-related activities.
• Maintain batch records and other documentations that are essential to be in compliance with ISO13485:2016.
• Manage laboratory equipment and supplies.
• Other projects or responsibilities may be required.

Minimum Qualifications

• Bachelor's degree in Life Science or related area
• 6-months of hands-on experience with bacterial and/or mammalian cell culture
• Extensive experience in suspended E. coli culture
• Experienced in purification and formulation of recombinant protein or antibody under ISO/GMP environment
• Capable of working cross-functionally with other departments and coordinating projects internally and externally
• Demonstrated ability to be detail-oriented, organized and work well in small work groups
• Able to perform basic computer operations such as MS Office and Outlook and other computer-based software
• Demonstrated ability to be detail-oriented, organized, and work well in small work groups

Preferred Qualifications

• Bachelor’s Degree in Life Science or related area with at least 1 year of working experience in related biotech protein or antibody product manufacturing
• Experienced in purification and formulation of recombinant protein or antibody under ISO/GMP environment
• Capable of working cross-functionally with other departments and coordinating projects internally and externally
• Demonstrated ability to be detail-oriented, organized, and work well in small work groups
• Able to perform basic computer operations such as MS Office and Outlook and other computer-based software
• Demonstrated ability to be detail-oriented, organized, and work well in small work groups

Work Environment & Physical Demands

Lab While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment. The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.