Biochemist I/II - Recombinant Protein Manufacturing

About the role

BioLegend is a global provider of health science solutions, technologies, expertise, and services for life sciences and diagnostics. This position is part of the MCBio group at BioLegend.

Responsibilities

• Prepare various buffers for experiments, including cell culture media.
• Perform transformation and culture of bacterial cells (1-50 liters).
• Process the culture supernatant/cell pellet/inclusion body, followed by extensive processing of the supernatant/cell pellet/inclusion body or diafiltration to extract or refold raw protein bulk.
• Support purifying recombinant protein by means of affinity tag.
• Support technical training for MFG procedures.
• Support activities related to audit readiness, CAPA, and NC investigation.
• Monitor processing by SDS-PAGE for varied protein production stages.
• Perform bulk processing (concentration, dilution, buffer change), purity analysis, and endotoxin removal.
• Maintain inventory and record for recombinant protein products.
• Assist in transferring products across different departments for QC or other inventory-related activities.
• Maintain batch records and other documentations that are essential to be in compliance with ISO13485:2016.
• Manage laboratory equipment and supplies.
• Other projects or responsibilities as required.

Requirements

• Bachelor's degree in Life Science or related area.
• 6 months of hands-on experience with bacterial and/or mammalian cell culture.
• Extensive experience in suspended E. coli culture.
• Experience in purification and formulation of recombinant protein or antibody under ISO/GMP environment.
• Capable of working cross-functionally with other departments and coordinating projects internally and externally.
• Demonstrated ability to be detail-oriented, organized, and work well in small work groups.
• Able to perform basic computer operations such as MS Office and Outlook and other computer-based software.

Qualifications

• Bachelor's Degree in Life Science or related area.
• At least 1 year of working experience in related biotech protein or antibody product manufacturing.

Skills

• Hands-on experience with bacterial and/or mammalian cell culture.
• Experience in purification and formulation of recombinant protein or antibody under ISO/GMP environment.
• Detail-oriented and organized work style.
• Basic computer operations such as MS Office and Outlook.

Benefits

We offer competitive and comprehensive benefits to our employees, including medical, dental, and vision insurance options, life and disability insurance, paid time-off, parental benefits, compassionate care leave, and a 401(k) with company match. Additionally, we offer an Employee Stock Purchase Plan.