Associate Scientist / Scientist, In Vitro HIV CAR-T

Key Responsibilities

• Generation of replication competent HIV reporter virus followed by virus titration.
• Evaluate different cell lines expressing CAR-T target for killing efficiency.
• Infection of primary activated PBMC / T-cells with subsequent flow analysis.
• Establish in vitro HIV challenge assays to quantify CAR-T mediated killing of PBMC.
• Perform and interpret multiparameter flow cytometry analyses to characterize CAR-T cell expression, target binding, killing, and analyze immune populations.
• Support molecular efforts such as lentivirus and CAR-T construct cloning, IVTs, etc.
• Integrate in vitro efficacy, cellular, and molecular readouts to guide program decisions.
• Troubleshoot experimental challenges and drive improvements in study design and execution.
• Maintain detailed and organized study records; contribute to study reports and presentations.

Qualifications

• PhD in Biology, Pharmacology, Immunology, Cancer Biology, or related field, with 4-6 years of relevant research or industry experience.
• Comfortable working with replication-competent HIV in an appropriate biosafety setting.
• Experience with culturing primary human PBMCs and isolating T-cell populations.
• Experience assessing in vitro tumor killing/efficacy and CAR-T expansion or persistence.
• Proficiency in multiparameter flow cytometry and flow cytometry data analysis.
• Experience with ddPCR, ELISA/MSD, or related molecular/protein assays is preferred.
• Strong experimental, design, analytical, troubleshooting, organizational, and communication skills.
• Meticulous attention to detail and strong documentation practices including use of electronic notebook for record keeping.
• A collaborative team player who fosters a culture of accountability, scientific rigor, and excellence and thrives in a fast-paced, results-oriented startup environment.
• A self-motivated, driven individual with a passion to learn and can-do attitude.

Pay

The salary range for this position is $112,000-$132,000.

Schedule

Not specified.

Benefits

Not specified.