Associate Scientist, Protein Science

About the role

The ideal candidate will bring hands-on experience with mammalian cell transfection, recombinant DNA techniques, and a working familiarity with downstream protein purification. They will support Alloy Discovery Services programs by executing protein science workflows central to antibody discovery, including transient transfection, recombinant DNA cloning, and immunogen design. They will contribute to protein expression at both high throughput and large scale as well as purification activities supporting the broader Protein Science team.

Responsibilities

• Perform small- to large-scale transient transfections in mammalian cell lines (e.g. CHO) to drive high yield biologics production
• Independently manage multiple cell culture lines maintaining proper tissue culture techniques
• Systematically evaluate and optimize transfection efficiency, cell viability, and expression protocols across scales to improve throughput, consistency, and overall yield
• Execute recombinant DNA workflows, including PCR, Gibson assembly, and plasmid scale-up
• Contribute to antibody construct engineering to support the production of multi-specific antibody candidates
• Support protein expression and purification of biologics using AKTA chromatography systems, including affinity, IEX, and SEC
• Analyze, interpret, and track deliverables in an electronic laboratory notebook in compliance with industry standards
• Coordinate project handoffs across functional teams to ensure seamless workflow continuity
• Present experimental data and findings during team and departmental meetings
• Ensure timely execution of project activities in alignment with priorities
• Maintain laboratory organization and ensure compliance with safety protocols

Qualifications

• MS with 2+ years or BS with 4+ years of hands-on research experience in molecular biology or a related discipline
• Extensive experience with transient expression of antibodies using mammalian expression systems from HTP to multi-liter scale, CHO preferred
• Deep technical understanding of various transfection methodologies (e.g., PEI, lipofectamine, or electroporation) and experience optimizing transfection efficiency and cell viability
• Proficiency in high-density suspension cell culture management, including scale-up strategies from deep-well plates and shaking flasks to bioreactors
• Hands-on experience monitoring cell culture health and metabolic profiles using analyzers such as Vi-CELL or equivalent instrumentation
• Proficiency in recombinant DNA techniques including PCR, cloning, restriction digestion, gel analysis, and plasmid purification at small and large scale
• Strong organizational skills with the ability to manage multiple projects and prioritize independently
• Good communication skills and willingness to learn and adapt as part of a dynamic, team-oriented environment