Associate Scientist II, in vitro Preclinical Development

About the Role

AbbVie is seeking a driven and innovative Associate Scientist II, in vitro Preclinical Development, to join the mRNA-tLNP group. The role focuses on developing and performing cell-based assays to advance mRNA-tLNP pipeline programs from early discovery through IND-enabling studies, with an emphasis on applications in oncology and autoimmune diseases.

Responsibilities

• Execute in vitro assays to evaluate mRNA-tLNP-mediated T-cell reprogramming, including assessment of cytotoxicity, T-cell effector function, and flow cytometry-based immune cell phenotyping.
• Isolate PBMCs and T cells from leukopaks.
• Use aseptic techniques to culture mammalian cells, including tumor cell lines, T cells, and PBMCs.
• Create stable tumor cell lines using techniques such as electroporation, viral delivery, and nanoparticle transfections.
• Perform routine tasks competently and independently, generating reliable and consistent results.
• Demonstrate skill in multiple techniques and appropriately modify protocols to suit research or development needs.
• Maintain and implement good laboratory practice, including accurate online laboratory notebook systems, instrument and reagent validation, and personal safety precautions.
• Work collaboratively in a cross-functional team environment.
• Organize and communicate data (written and oral) and participate in project team meetings.

Requirements

• Bachelor’s Degree (or equivalent education) with typically 3 or more years of experience, or a Master’s Degree (or equivalent education) in immunology, cell and molecular biology, or a related field.
• Previous experience in chimeric antigen receptor (CAR) production and evaluation is desirable.
• Experience with aseptic/sterile techniques for culturing mammalian cells, including cell lines, T cells, and PBMCs.
• Experience with viral and non-viral gene transfer techniques to engineer mammalian cells.
• Experience designing and executing assays to assess T cell function, including cytotoxicity, activation, proliferation, and cytokine profiling.
• Proficiency in flow cytometry and data processing.
• Experience designing and executing plate-based assays, including ELISA and Luminex/MSD.
• Experience with scientific data analysis software, including CellEngine and Tableau, is highly preferred.

Benefits

• Comprehensive package of benefits, including paid time off (vacation, holidays, sick), medical/dental/vision insurance, and 401(k) for eligible employees.
• Eligibility to participate in short-term incentive programs.