Viral Vector Engineer

Key Responsibilities

• Perform cloning of large viral genomes, design and run PCR, qPCR, RT-PCR assays to quantify viral transgene and transcription
• Transfect primary cells, immortalized cells, and animal tissues
• Design, manufacture, purify, characterize, validate and test a range of viral vectors, at small and large scale
• Absess efficiency of workflows through cellular assays and NGS sequencing approaches
• Extract DNA, RNA, protein from animal tissue
• Conduct experiments and work on research problems
• Maintain excellent electronic laboratory records
• Work on project milestones
• Attend lab meetings, prepare reports and presentations on the progress of projects

Required qualifications

• Bachelor's, Master's, or PhD in biological sciences, bioengineering, molecular biology, genetics, or any science related field, or equivalent experience
• Experience with cloning, transfecting, producing, purifying, characterizing, validating and testing of a range of viral vectors including adenovirus, lentivirus, AAV
• Experience with ultracentrifugation for viral purification and resin and column based viral purification methods
• Strong aseptic techniques and GLP procedures
• Experience in genome engineering, cloning, DNA amplification, PCR, NGS, transfections, and other molecular biology skills
• Exceptional organizational skills with the ability to effectively manage time and priorities
• Strong written and oral communication skills, as well as creative problem-solving skills
• Curious, eager, and hardworking

Preferred qualifications

• Experience with viral capsid engineering by rational design or directed evolution
• Extensive experience with and knowledge of adenoviral vectors
• Experience with viral capsid library engineering

Pay

Base salary is expected to be $90-130K depending on experience, with additional bonus compensation, equity compensation, and benefits including health insurance, dental insurance, vision insurance, and 401(k).