Systems Validation Scientist II

Job Summary

At Client Biosciences, our purpose is to help all people live healthy lives. We are a world leader bringing innovative tools to research and clinical laboratories in basic research, drug discovery and development, biopharmaceutical production and disease management. We develop technology that enables faster diagnosis and better clinical outcomes.

Responsibilities

• Maintain the health, expansion, and inventory of cell lines
• Perform cryopreservation and thawing of mammalian cells
• Purify leukocytes from mammalian-derived samples (i.e., venous blood) via different density-gradient centrifugation methods and RBC lysis protocols
• Stain cells in tubes and plates for intracellular and extracellular antigens
• Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents
• Count cells via hemocytometer and/or proprietary cell counters
• Prepare buffers, media, and other reagents
• Maintain inventory of critical laboratory supplies (consumables and reagents) in the lab
• Attend meetings, as needed.

Qualifications

• A minimum of a bachelor’s degree in Microbiology, Biology, Biochemistry, Immunology, or a related field
• At least 2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines
• Best practice experience doing antibody methods for antigen labeling (e.g., flow cytometry, western blots, ELISA, ELISPOT)
• Experience working with primary cells derived from various mammalian species
• Proficient with phase-contrast and brightfield microscopy
• Excellent aseptic technique
• Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies
• Ability to calculate basic laboratory calculations
• Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency
• Ability to read and understand Standard Operating Procedures
• Ability to interact with researchers for delivery of cells
• Ability to maintain a clean work area
• Knowledge of GLP/GDP guidelines
• Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
• Excellent written and oral communication skills