Staff Research Associate - Pediatric Oncology

About the Lab

The Huang Lab at UCSF (https://huanglabucsf.com/) investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include:

• Determining the biochemical mechanisms governing growth, survival, and differentiation of leukemia cells.
• Identifying genes and biochemical pathways that are regulated differentially in leukemia versus normal cells.
• Measuring the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro.
• Harvesting murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients.
• Transplanting genetically modified hematopoietic cells into recipient mice.
• Performing genetic analysis on mouse tissues.
• Administering chemotherapeutic agents, radiation, and new experimental treatments to mice.

Responsibilities

• Cell and tissue culture.
• Biochemical assays (western blotting, immunoprecipitation, qPCR).
• DNA and RNA extraction and manipulation.
• PCR and agarose gel electrophoresis.
• Generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells.
• Flow cytometry.
• Maintenance of accurate and complete records.
• Organization and maintenance of mouse colonies, including computerized record-keeping, breeding, monitoring, and genotyping.
• Experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues).

Requirements

Required Qualifications: BA/BS in a related science or an equivalent combination of education and experience.

Preferred Qualifications:

• Expertise in breeding and maintaining laboratory mice.
• Molecular biology skills including DNA/RNA extraction and PCR amplification.
• Tissue culture skills including passaging cell lines.
• Biochemistry skills including Western blotting.