Sr. Scientist, Lentivirus Upstream Processing

Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases. We are developing advanced cell therapies across a diverse array of technology platforms, including autologous and allogenic chimeric antigen receptor T-cell, T-cell receptor (TCR-T), and natural killer (NK) cell-based immunotherapy.

About the role

The Senior Scientist, Lentivirus Upstream Process Development, will be a core technical contributor responsible for designing, executing, and optimizing upstream production processes for lentiviral vectors (LVV) within our innovative pipeline. This role focuses on delivering high-quality, high-titer vector products to support LVV generation and other advanced cell therapy programs. The successful candidate will apply technical expertise in upstream processing (USP)—including seed train optimization, mammalian cell culture (HEK293/HEK293T), large-scale transient transfection, and bioreactor scale-up—to ensure processes are robust, scalable, and phase-appropriate for GMP manufacturing.

Responsibilities

• Design and execute experimental strategies for lentiviral vector upstream production.
• Perform development and optimization of upstream processes, including seed train expansion, suspension mammalian cell culture (HEK293/HEK293T), plasmid transient transfection optimization, and feed batch/perfusion bioprocessing in stir-tank and rocker bioreactors.
• Support the establishment and validation of bioreactor scale-up and scale-down models to ensure process comparability and predictability.
• Support the translation of bench-scale processes to GMP manufacturing, assisting in technology transfer and providing troubleshooting support during clinical production runs.
• Execute study protocols to define operating parameters, feeding strategies, and performance limits; maintain accurate documentation for USP data.
• Communicate technical findings, issues, and potential delays to project leads in a timely manner.
• Collaborate with cross-functional team members, including CMC Project Management and other technical leads, to support project goals.
• Apply scientific knowledge and technology to optimize vector titer, specific productivity, and quality.
• Support team activities by providing technical guidance to junior staff, fostering a culture of scientific rigor and continuous improvement.
• Author and review technical documents, including development reports, SOPs, and relevant sections for regulatory submissions (IND).
• Work closely with Downstream Process Development and Analytical Development teams to ensure integrated process outcomes.
• Support tech transfer activities to/from other Legend sites or CDMOs.
• Ensure compliance with all internal and regulatory requirements.

Requirements

• Advanced degree (PhD preferred, or MS) in Chemical Engineering, Bioengineering, or a related field.
• Experience: 5-8 years (PhD) or 8+ years (MS) of experience in process development or manufacturing within a biotech or pharmaceutical setting.
• Hands-on experience in mammalian cell culture (HEK293, HEK293T), seed train expansion, feeding strategy, and transient transfection optimization specifically for viral vectors (Lentivirus preferred).
• Demonstrated proficiency in developing and implementing bioreactor scale-up and scale-down models.
• Solid understanding of GMP manufacturing requirements and CMC strategies for early-phase clinical trials.
• Direct experience with cell and lentiviral vector technologies within the current emerging field.
• Working knowledge in engineering modeling, mass transfer, and bioprocess optimization.
• Strong interpersonal and communication skills; ability to maintain focus and collaborate effectively during challenging development timelines.
• Analytical and creative thinking.
• Effective troubleshooting skills.
• Preferred experience in handling large-scale (>50L, e.g., 200L/500L) upstream bioreactor processes.
• Language(s): English. Mandarin is a plus.

Pay

Base pay range: $127,313 - $167,099 USD. Performance-based bonus and/or equity is available to employees in eligible roles.

Benefits

• Medical, dental, and vision insurance.
• 401(k) retirement plan with a company match that vests fully on day one.
• Eight weeks of paid parental leave after three months of employment.
• Paid time off policy including vacation time, personal time, sick time, floating holidays, and eleven company holidays.
• Flexible spending and health savings accounts.
• Life and AD&D insurance, short- and long-term disability coverage.
• Legal assistance and supplemental plans such as pet, critical illness, accident, and hospital indemnity insurance.
• Voluntary commuter benefits, family planning and care resources, well-being initiatives, and peer-to-peer recognition programs.

Note: These benefits are offered exclusively to permanent full-time employees. Contractors are not eligible for benefits through Legend Biotech.