Senior Research Scientist

Duties

• Independently leading scientific research projects and supervising trainees and research activities
• High-level molecular biology, including sequencing and sample/library preparation
• Working with murine models of immune dysregulation, including asthma, neuroinflammation, autoinflammation, infection, and cardiovascular disease
• Immune cells functional assays
• Cloning and transfection
• Screwing primary patient cells for disease-causing mutations

Requirements

• PhD degree (or foreign equivalent degree) in Biology, Immunology or a related field plus three (3) years of experience in a related position
• Experience must include three (3) years working with:
• Human immunology research, including primary human immune cell isolation, culture, stimulation, gene editing, and functional analysis
• Murine models of inflammation, allergy, and atherogenesis, including designing and conducting in vivo experiments and processing a wide range of tissues (aorta, lungs, GI, skin) for immunologic analysis, histological analysis, and specialized staining (i.e. aortic plaque staining)
• Multiparameter flow cytometry, including experimental design, panel development, compensation, gating strategies, and data analysis
• Molecular biology techniques, including nucleic acid extraction, qPCR, ddPCR, RNA-seq
• Gene-expression manipulation using lentiviral systems, including viral particles production and transduction for stable knockdown, knockout, or overexpression
• Functional in vitro assays with mammalian cell lines, including evaluation of cell activation, viability, proliferation, cytokine production, etc
• Handling, shaving, and injecting (IV, IP, intradermal) mice and processing broad range of tissues

Qualifications

Must have a PhD degree (or foreign equivalent degree) in Biology, Immunology or a related field plus three (3) years of experience in a related position.

Skills

Experience must include three (3) years working with:

• Human immunology research, including primary human immune cell isolation, culture, stimulation, gene editing, and functional analysis
• Murine models of inflammation, allergy, and atherogenesis, including designing and conducting in vivo experiments and processing a wide range of tissues (aorta, lungs, GI, skin) for immunologic analysis, histological analysis, and specialized staining (i.e. aortic plaque staining)
• Multiparameter flow cytometry, including experimental design, panel development, compensation, gating strategies, and data analysis
• Molecular biology techniques, including nucleic acid extraction, qPCR, ddPCR, RNA-seq
• Gene-expression manipulation using lentiviral systems, including viral particles production and transduction for stable knockdown, knockout, or overexpression
• Functional in vitro assays with mammalian cell lines, including evaluation of cell activation, viability, proliferation, cytokine production, etc
• Handling, shaving, and injecting (IV, IP, intradermal) mice and processing broad range of tissues

Benefits

N/A

Pay

TBD

Schedule

N/A

Benefits

N/A

Application

Apply at , #26003015.

Talent Acquisition

The University of Pittsburgh is an equal opportunity employer.