Scientist – Molecular Cloning & Automation

Job Description

We are seeking a skilled Scientist – Molecular Cloning & Automation to lead plasmid design, DNA assembly, and scalable workflow development. You will apply advanced cloning strategies and build automated solutions to support high-throughput construct generation, ensuring quality, efficiency, and on-time delivery across multiple programs.

Job Responsibilities

• Design and execute end-to-end plasmid workflows, translating protein or guide RNA sequences into verified, production-ready constructs
• Select and apply appropriate cloning strategies (e.g., Golden Gate, Gibson Assembly, restriction-ligation) based on construct complexity
• Develop, implement, and scale automated workflows for high-throughput plasmid production, including:
• Liquid handling platforms (Hamilton STAR, Opentrons, Beckman Biomek)
• Automated colony picking systems (e.g., QPix)
• High-throughput miniprep processes
• Establish and manage tiered QC pipelines (colony PCR, restriction digest, Sanger and/or long-read sequencing) while maintaining accurate construct documentation
• Oversee CRO and vendor relationships for outsourced cloning and gene synthesis, including construct submission, timeline management, and QC review
• Partner cross-functionally to deliver constructs on time, managing multiple concurrent requests with clear, proactive communication

Qualifications

• Required Qualifications:
• B.S. with 5+ years, M.S. with 3+ years, or Ph.D. with 2+ years of relevant industry experience in Molecular Biology, Biochemistry, Bioengineering, or a related field
• Expert proficiency in Golden Gate, Gibson Assembly, and restriction-ligation cloning, with the ability to select optimal strategies
• Strong knowledge of plasmid biology, including origins of replication, selection markers, promoter design, codon optimization, and management of recombination-prone sequences
• Hands-on PCR experience, including polymerase selection (e.g., Q5, Phusion), primer design, and troubleshooting challenging amplicons.
• Experience managing CRO/vendors for gene synthesis or outsourced cloning
• Proficiency with Benchling or similar ELN for construct tracking and QC documentation
• Familiarity with sequence design and visualization tools (e.g., SnapGene, Geneious)
• Proven ability to manage multiple simultaneous cloning projects and deliver high-quality constructs on timeline
• Strong communication skills with a collaborative, cross-functional mindset
• Preferred Qualifications:
• Experience programming, validating, and troubleshooting liquid handling platforms (e.g., Hamilton, Beckman, Tecan, Opentrons)
• Familiarity with automated colony picking and high-throughput plasmid prep workflows
• Experience with pooled oligo cloning, including library design, bias mitigation, and NGS-based QC analysis
• Background in lentiviral or pooled library cloning workflows
• Experience with long-read sequencing technologies (PacBio or Nanopore)
• Exposure to gene therapy applications (AAV, LVV, mRNA, CAR-T) and downstream construct use
• Experience supporting IVT template design or mRNA production workflows

Additional Information

• What to Expect in the Hiring Process:
• 10-15 Minute Phone Interview with Region Recruiter
• 45-60 Minute Virtual Interview with Manager and/or Group Leader
• 30 Minute In-Person Onsite Meeting with the Team
• Additional Details:
• The position is Full Time, Monday-Friday, 8am-5pm.
• Candidates currently living within a commutable distance of Boston, MA are encouraged to apply.
• Excellent full-time benefits include:
• Comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays

Pay

Hourly rate is between $45.00-$48.00, depending on education and experience

Schedule

Monday-Friday, 8am-5pm

Benefits

• Comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays

Additional Details

• The position is Full Time, Monday-Friday, 8am-5pm.
• Candidates currently living within a commutable distance of Boston, MA are encouraged to apply.

About the Role

The work we do at Eurofins Professional Scientific Services matters, and so do our employees. At Eurofins PSS, we put a focus on your professional growth. We provide our employees the ability to break into the biopharmaceutical industry and grow from the bottom up. We have the supportive teams and tools available to invest in you while also affording you work-life balance and a competitive benefits package.

Requirements

None specified

Qualifications

• Required Qualifications:
• B.S. with 5+ years, M.S. with 3+ years, or Ph.D. with 2+ years of relevant industry experience in Molecular Biology, Biochemistry, Bioengineering, or a related field
• Expert proficiency in Golden Gate, Gibson Assembly, and restriction-ligation cloning, with the ability to select optimal strategies
• Strong knowledge of plasmid biology, including origins of replication, selection markers, promoter design, codon optimization, and management of recombination-prone sequences
• Hands-on PCR experience, including polymerase selection (e.g., Q5, Phusion), primer design, and troubleshooting challenging amplicons.
• Experience managing CRO/vendors for gene synthesis or outsourced cloning
• Proficiency with Benchling or similar ELN for construct tracking and QC documentation
• Familiarity with sequence design and visualization tools (e.g., SnapGene, Geneious)
• Proven ability to manage multiple simultaneous cloning projects and deliver high-quality constructs on timeline
• Strong communication skills with a collaborative, cross-functional mindset
• Preferred Qualifications:
• Experience programming, validating, and troubleshooting liquid handling platforms (e.g., Hamilton, Beckman, Tecan, Opentrons)
• Familiarity with automated colony picking and high-throughput plasmid prep workflows
• Experience with pooled oligo cloning, including library design, bias mitigation, and NGS-based QC analysis
• Background in lentiviral or pooled library cloning workflows
• Experience with long-read sequencing technologies (PacBio or Nanopore)
• Exposure to gene therapy applications (AAV, LVV, mRNA, CAR-T) and downstream construct use
• Experience supporting IVT template design or mRNA production workflows

Skills

• Expert proficiency in Golden Gate, Gibson Assembly, and restriction-ligation cloning
• Strong knowledge of plasmid biology
• Hands-on PCR experience
• Experience managing CRO/vendors for gene synthesis or outsourced cloning
• Proficiency with Benchling or similar ELN for construct tracking and QC documentation
• Familiarity with sequence design and visualization tools
• Proven ability to manage multiple simultaneous cloning projects
• Collaborative, cross-functional mindset

Benefits

• Comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays

Pay

Hourly rate is between $45.00-$48.00, depending on education and experience

Schedule

Monday-Friday, 8am-5pm

Location

Boston, MA

Equal Opportunity Employer

Eurofins USA PSS Insourcing Solutions is a Disabled and Veteran Equal Employment Opportunity employer.