Research Technologist II

Job Summary and Responsibilities

• Perform standard laboratory procedures with some independence, under the general supervision of a PI or Research Technician.
• Execute experimental protocols, collect/record data, and maintain lab equipment.
• Provide guidance to students.
• Receive and manage biospecimens including tumor tissue, cerebrospinal fluid (CSF), and blood in compliance with CLIA-regulated protocols.
• Isolate cell-free DNA from CSF and extract genomic DNA from tumor tissue and peripheral blood.
• Isolate circulating cells from CSF and perform tissue dissociation for downstream applications.
• Prepare single-cell RNA sequencing libraries using the 10x Genomics platform from tumor and CSF-derived cells.
• Generate DNA sequencing libraries from tumor tissue and blood.
• Maintain rigorous accurate documentation of experimental workflows in accordance with established protocols and regulatory standards.
• Contribute to a collaborative research environment through participation in lab meetings, seminars, and journal clubs.
• Keeps accurate records of studies conducted with minimal supervision.
• Performs basic data analysis and processing as required by senior laboratory personnel.
• Creates, maintains, and updates lab's internal procedure manuals.

Job Requirements

• Required: Bachelor’s degree in biological sciences or a related field, two (2) years prior experience in research, clinical, or other scientific environment, prior laboratory research experience with demonstrated proficiency in cell culture, molecular biology techniques (e.g. PCR, qPCR), and/or sequencing library preparation, strong analytical and problem-solving skills with the ability to learn and execute new techniques independently, excellent attention to detail, organization, and time management, strong written and verbal communication skills.
• Prior experience: Four (4) years of prior experience in a research, clinical, or other scientific environment, background in cell biology, neuroscience, or oncology, familiarity with CLIA-regulated workflows and liquid biopsy methodologies, experience with single-cell RNA sequencing and next-generation sequencing platforms.

Additional Information

• Two to three letters of recommendation may be requested.

Where You'll Work

• Hello Humankindness: Join a Legacy of Healing at Saint Joseph’s Hospital and Medical Center (SJHMC)
• Barrow Neurological Institute (BNI): A world-renowned center for groundbreaking neurological research and innovative treatments.
• Norton Thoracic Institute (NTI): A national leader in advanced thoracic care with pioneering surgical techniques.
• Level I Trauma Center: One of Arizona's busiest ERs, providing immediate, comprehensive care for critically injured patients 24/7.

Pay Range

$20.91 - $29.54 /hour