Research Scientist III; Protein Science

Responsibilities

• Conduct protein purification with FPLC including affinity, ion exchange, HIC, etc.
• Conduct protein purification from E. coli, mammalian and/or insect cells.
• Run protein characterization including: HPLC, SDS-PAGE, native PAGE, western blot, DLS, and endotoxin assay.
• Be responsible for cell culture maintenance and transfections including: CHO, HEK 293, Sf9, and Sf21.
• Run molecular cloning including: PCR, DNA digestion, ligation, and mutagenesis.
• Present data (written and/or oral) to clients as necessary.
• Collaborate and provide specific technical expertise to multidisciplinary teams such as assay, cell biology, medicinal chemistry teams (internal and external) for preclinical drug development.
• Conduct laboratory operations in a safe manner. Maintain familiarity with the Chemical Hygiene Plan. Exhibit safety awareness and safe work practices.
• Follow responsible actions regarding chemical disposal. Maintain compliance with all regulations at the federal, state, and local levels, as well as all appropriate SOPs.
• Use and maintain equipment in a neat and orderly manner. Report any malfunction immediately to supervisor. Repair minor problems.
• Maintain service and calibration records.
• Submit complete, documented, and legible reports of testing. Ensure that data is properly filed and accessible for review in accordance with appropriate SOPs.
• Recommend and implement methods to increase the quality of products and/or services.
• Discuss routinely with supervisor the status of assigned programs and potential problems.
• Organize work time so that multiple activities run concurrently. Use time efficiently to accomplish immediate task and perform other needed activities.
• Manage workload and time to enable the incumbent to perform multiple projects effectively, and ensure all necessary paperwork is completed on a timely basis.
• Participate in self-development activities and training of others.

Qualifications

• Bachelor’s degree with a major in Biology or Biochemistry, and at least five to eight years of relevant experience, or equivalent industrial experience OR
• Master’s degree with a major in Biology or Biochemistry, and at least three to six years of relevant experience, or equivalent industrial experience OR
• Ph. D. with a major in Biology or Biochemistry, and at least zero to three years of relevant experience, or equivalent industrial experience.
• Extensive experience with cell culture and recombinant protein purification.
• Preferred: Prior experience with protein purification systems such as AKTA, NGS, or similar.
• Skill of planning and organization of multiple projects simultaneously.
• Ability of scientific data presentation and client communication.
• Prior experience with molecular cloning and protein analysis.
• Prior experience of purification of challenging proteins and troubleshooting skills.

Other Qualifications

• Must pass a background check.
• Must pass a drug screen.
• May be required to pass Occupational Health Screening.

Pay

Pay Range: $70,000/yr.