Research Scientist, Gene Editing Core

Mass General Brigham is a not-for-profit healthcare system supporting patient care, research, teaching, and community service across a wide range of professionals.

About the role

The Gene and Cell Therapy Institute (GCTI) is a newly created system-wide institute under Mass General Brigham, fostering a world-class gene and cell therapy program that leverages groundbreaking scientific research across the MGB system. The Gene Editing Core (GEC) serves as a catalyst in the gene therapy revolution, providing comprehensive support to transform genetic discoveries into therapeutic realities — from strategic consulting and experimental design to cutting-edge assay development and high-throughput screening. We are seeking a highly skilled Research Scientist to support and drive genome editing and CRISPR perturbation projects. The successful candidate will develop and execute advanced gene editing workflows, CRISPR perturbation screening assays, and off-target analysis methods, working alongside world-class scientists and utilizing cutting-edge technologies to advance the next generation of genetic therapies. The position offers exposure to high-impact collaborations with academic and industry partners, along with access to professional development opportunities including conference participation and networking.

Responsibilities

• Develop and optimize CRISPR-based genome editing techniques for various cell types, including iPSCs and primary cells
• Perform molecular biology assays, including molecular cloning, PCR, ddPCR, RT-qPCR, NGS library preparation, and quality control assessments
• Conduct off-target analysis using next-generation sequencing (NGS)-based methods to assess genome editing outcomes
• Lead and execute experiments for genetic perturbation studies, including pooled and arrayed CRISPR screens
• Work closely with internal and external collaborators to design, troubleshoot, and optimize gene editing experiments
• Maintain detailed and well-organized experimental records in electronic lab notebooks (ELNs)
• Stay current with emerging technologies in genome editing, CRISPR perturbation, and functional genomics
• Train and mentor junior staff and research technicians in gene editing workflows and molecular biology techniques
• Contribute to scientific publications and presentations at internal and external meetings
• All other duties as assigned

Requirements

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field
• Strong expertise in molecular biology techniques, including PCR, cloning, qPCR, ddPCR, and NGS library preparation
• Extensive experience with CRISPR-based genome editing and perturbation screening technologies
• Hands-on experience with molecular cloning methods, including Gibson Assembly and Golden Gate cloning, particularly for CRISPR library generation
• Familiarity with off-target analysis techniques and NGS
• Ability to analyze sequencing data and interpret gene editing efficiency and specificity
• Excellent organizational skills, attention to detail, and ability to manage multiple projects simultaneously
• Strong written and verbal communication skills

Preferred Qualifications

• Experience with prime editing and base editing workflows
• Expertise in CRISPR screening approaches, including CRISPR KO, CRISPRi, and CRISPRa
• Experience working in a core facility or industry setting providing genome-editing services
• Familiarity with bioinformatics tools for analyzing NGS data (e.g., Python, R, or Bioconductor)
• Hands-on experience with iPSC culture and differentiation

Pay

The salary range for this position is $80,000 to $110,000 annually. Base pay is determined by skills, relevant experience, education, certifications, and other essential factors. In addition to competitive base pay, comprehensive benefits, career advancement opportunities, and recognition programs are offered.

Schedule

Full-time, 40 scheduled weekly hours. Day shift. Regular employee type.

Location

Onsite at 149 Thirteenth Street, Building 149, Charlestown Navy Yard.