Research Lab Tech

About the role

A research lab within Brigham and Women’s Cardiovascular Medicine division is looking for a highly motivated technical research assistant I. The lab uses both in vitro (human ESC/iPSC) and in vivo (zebrafish) techniques to model various cardiovascular disorders, including atrial fibrillation, hypertrophic cardiomyopathy, atherosclerosis, and arteriovenous malformations. By integrating transcriptional, (epi)genetic, metabolic, and phenotypic profiling at whole organism, tissue, and single cell-level, the lab aims to understand the earliest molecular events characterizing disease onset, follow their dynamic changes over time, and to identify disease biomarkers. The lab also uses these zebrafish models to expedite drug discovery in cardiovascular medicine.

Responsibilities

• Perform a variety of laboratory experiments under general supervision, including molecular and cellular techniques such as PCR, microdissection, western blotting, immunofluorescence microscopy, DNA purification, and quantitative RT-PCR
• Maintain zebrafish colonies
• Prepare and maintain cell and tissue cultures
• Prepare laboratory reagents, chemicals, instruments, and equipment
• Perform chemical informatics
• Collaborate with a team of research assistants and post docs to meet project goals in a fast-paced and dynamic wet lab setting
• Help to establish new and modify existing research techniques
• Coordinate with core facilities and collaborating labs
• Perform time-lapse microscopy and automated image processing
• Meticulously record procedures and results in an electronic lab notebook; follow required safe practices and comply with institutional policies and procedures
• Discuss progress of work regularly with supervisors
• Work some nights and weekends when needed (overtime will apply)
• Perform other duties as assigned

Qualifications

• BA/BS in biology, chemistry, or closely related field
• 0–1 years of experience

Preferred

• Prior laboratory research experience
• Prior experience working with zebrafish

Additional Skills/Abilities/Competencies

• Ability to perform a variety of laboratory experiments related to molecular and cellular biology
• Exceptional ability to participate in team-based, high-throughput laboratory activities
• Adaptability to changing experimental protocols and willingness to participate in setting up new protocols
• Strong organizational skills; detail oriented
• Ability to maintain an accurate and complete electronic lab notebook and electronic logs of stored samples
• Knowledge of sterile techniques including ability to use pipettes, test tubes, culture dishes and flasks in a cell culture hood
• Excellent analytical and communication skills
• Interest in and willingness to accept new responsibilities
• Ability to lift 20 lbs
• High personal standards for quality, accuracy, and completeness of experimental work

Schedule

Scheduled Weekly Hours: 40

Work Shift: Day (United States of America)

Pay

Pay Range: $21.00 – $29.01/Hourly