Research Lab Specialist Inter
About the role
thenwith the summary. Responsibilities: there is a "Responsibilities* Include But Are Not Limited To" then bullet list items. We'll extract those. Requirements: There's "Required Qualifications*" and "Associate Requirements". Those are qualifications. We'll treat as Requirements section maybe. Also there are "Desired Qualifications*". Those could be Qualifications or Skills. The instruction: Use headings for sections like About the role, Responsibilities, Requirements, Qualifications, Skills, Benefits, Pay, Schedule. So we need to decide where each piece fits. We have: - Job Summary (About the role) - Responsibilities (list) - Required Qualifications (list) - Associate Requirements (list) - maybe also part of Requirements. - Desired Qualifications (list) - could be Skills or Qualifications. - Modes of Work (maybe not needed) - Underfill Statement (maybe not needed) - Background Screening (maybe not needed) - Application Deadline (maybe not needed) - U-M EEO Statement (boilerplate to remove) We should remove boilerplate: Underfill Statement, Background Screening, Application Deadline, U-M EEO Statement, Modes of Work maybe not needed unless it's specific content about hybrid/remote. It says "Modes of Work Positions that are eligible for hybrid or mobile/remote work mode are at the discretion of the hiring department. Work agreements are reviewed annually..." That's generic; maybe we can drop as boilerplate. No specific benefit/pay/schedule info given. There's mention of rotating weekend/holiday feeding schedule (that's schedule). So we can include a Schedule section with that detail. Also there is "Preference will be given to applicants who seek at least 2 years of commitment to this position." That's maybe a requirement or preference; could be part of Requirements. Also "As this position includes opportunity for career growth, we particularly encourage those seeking a long-term position." That's maybe not specific. We need to preserve all substantive information; do not invent or summarize away detail. So we need to include all substantive info from the posting, but we can omit boilerplate. Let's extract substantive content: Job Summary paragraph: "The Srinivasan Laboratory is looking to fill a position for Research Lab Specialist Intermediate The overarching goals of the laboratory are to study the basic biology and mechanisms underlying neurodegenerative diseases and to discover novel therapeutics for future clinical development. In particular, the Srinivasan Laboratory is very interested in targeting the dysfunctional cerebellar circuitry that drives the dominantly inherited Spinocerebellar Ataxias (SCAs). The desired candidate will have hands-on experience with human pluripotent stem cell (iPSC/ESC) culture, along with skills in molecular biology, cell biology, and biochemistry. Those with stem cell culture experience will be prioritized in the interview process, though previous mammalian cell culture with extensive sterile technique may be appropriate. The successful candidate will work alongside Dr. Srinivasan and existing lab members to maintain and expand human iPSC lines, carry out directed differentiation into cerebellar and other disease-relevant neurons, generate and characterize gene-edited stem cell lines, and design, perform, and interpret experiments on channel function and pharmacology in these models. In addition, the successful candidate will supervise and help train other members of the lab (including undergraduates, graduate students, and other technicians), including instruction in sterile technique and stem cell culture. Applicants who have independently maintained pluripotent stem cell cultures are especially encouraged to apply. Because stem cell lines require uninterrupted care, the position includes participation in a rotating weekend and holiday feeding schedule shared across the lab. Preference will be given to applicants who seek at least 2 years of commitment to this position. As this position includes opportunity for career growth, we particularly encourage those seeking a long-term position." That's a lot; we can keep as prose. Responsibilities list: - Laboratory maintenance - Maintain, expand, and cryopreserve human pluripotent stem cell (iPSC/ESC) lines, including feeder-free culture, passaging, thawing, and routine quality control - Perform directed differentiation of stem cell lines into neurons and other disease-relevant cell types - Keep detailed culture and line-provenance records and share in a rotating weekend/holiday feeding schedule - Conduct experiments - Analyze data - Train users in equipment operation and standard techniques - Participate in periodic research discussions (weekly lab meeting) - Present data and plans for new experiments - Collaborate with colleagues to achieve short- and long-range plans - Help assemble manuscripts, chapters, and research abstracts - Additional duties as assigned, such as lab ordering (bi-weekly). We need to format as
- items. Some items have sub-bullets; we can keep them as separate
- with subtext.
Required Qualifications:
- Intermediate Masters degree in biological sciences, including molecular biology, biochemistry or a related discipline
- 3-4 years of wet-laboratory experience including direct experience culturing human pluripotent stem cells (iPSC or ESC): passaging, feeding, freezing and thawing, and monitoring cultures for spontaneous differentiation and contamination.
Associate Requirements (alternative path):
- bachelors degree in the biological sciences, including molecular biology, biochemistry or a related discipline
- 1-2 years of laboratory experience which could include classroom laboratory experience or employment
- Hands-on experience with sterile mammalian cell culture, including independent day-to-day maintenance of continuous cultures
Desired Qualifications:
- Two or more years of dedicated human pluripotent stem cell culture experience in an academic or industry laboratory
- Experience with directed differentiation protocols and with iPSC-derived neuronal, organoid cultures
- Experience with live-cell and confocal imaging and with flow cytometry cultured cells
- Experience supporting plate-based or high throughput screening workflows, including 24-96-well culture, liquid handling, and assay quality control
- Familiarity with basic techniques in biomedical research, DNA/RNA extraction and purification, quantitative real-time PCR, Western blotting, and fluorescence microscopy
- Experience using CRISPR techniques in cell culture, particularly gene editing of iPSC lines, single-cell cloning, and screening and validation of edited clones
- Experience with tissue processing and immunohistochemistry in fixed tissues
Also mention: "Preference will be given to applicants who seek at least 2 years of commitment to this position." Could be part of Requirements or maybe a separate note; we can include in Requirements as a bullet.
Also "As this position includes opportunity for career growth, we particularly encourage those seeking a long-term position." Could be a note but not specific; maybe we can omit as it's vague.
Schedule: rotating weekend and holiday feeding schedule shared across the lab. That's schedule info.
Benefits: none specific given. Pay: none.
Thus we will have sections: About the role, Responsibilities, Requirements, Qualifications (maybe Desired Qualifications), Skills (maybe we could put desired qualifications under Skills), Schedule.
But we need to avoid thin sections. Let's combine Desired Qualifications into Skills section. Or we could have Qualifications (required) and Skills (desired). The instruction: Use headings for sections like About the role, Responsibilities, Requirements, Qualifications, Skills, Benefits, Pay, Schedule. So we can have both Requirements and Qualifications and Skills. But we need to avoid filler. We have content for Requirements (required qualifications). We have content for Desired Qualifications (skills). We could put those under Skills.
Thus:
- About the role: job summary paragraph.
- Responsibilities: list.
- Requirements: required qualifications + associate requirements + preference for 2-year commitment.
- Skills: desired qualifications list.
- Schedule: rotating weekend/holiday feeding schedule.
We should not include Benefits, Pay if no specific content.
Now we need to output HTML fragment only, no /. Use
for headings,
for prose,
- for lists.
We need to ensure we preserve all substantive information. Let's craft.
About the role: we need to include the whole job summary paragraph as prose. Might be long but okay.
We'll wrap in
. Responsibilities: we need to capture each bullet. Let's list them as separate
- . For sub-items like under Laboratory maintenance we can keep as separate
- with the details. Let's extract: Laboratory maintenance - Maintain, expand, and cryopreserve human pluripotent stem cell (iPSC/ESC) lines, including feeder-free culture, passaging, thawing, and routine quality control - Perform directed differentiation of stem cell lines into neurons and other disease-relevant cell types - Keep detailed culture and line-provenance records and share in a rotating weekend/holiday feeding schedule Conduct experiments Analyze data Train users in equipment operation and standard techniques Participate in periodic research discussions (weekly lab meeting) Present data and plans for new experiments Collaborate with colleagues to achieve short- and long-range plans Help assemble manuscripts, chapters, and research abstracts Additional duties as assigned, such as lab ordering (bi-weekly). We'll make each as
- . For the ones with sub-bullets we can just include them as separate
- but maybe we want to keep grouping? Not required. Thus list:
- Maintain, expand, and cryopreserve human pluripotent stem cell (iPSC/ESC) lines, including feeder-free culture, passaging, thawing, and routine quality control
- Perform directed differentiation of stem cell lines into neurons and other disease-relevant cell types
- Keep detailed culture and line-provenance records and share in a rotating weekend/holiday feeding schedule
- Conduct experiments
- Analyze data
- Train users in equipment operation and standard techniques
- Participate in periodic research discussions (weekly lab meeting)
- Present data and plans for new experiments
- Collaborate with colleagues to achieve short- and long-range plans
- Help assemble manuscripts, chapters, and research abstracts
- Additional duties as assigned, such as lab ordering (bi-weekly) Now Requirements: include: - Intermediate Masters degree in biological sciences, including molecular biology, biochemistry or a related discipline - 3-4 years of wet-laboratory experience including direct experience culturing human pluripotent stem cells (iPSC or ESC): passaging, feeding, freezing and thawing, and monitoring cultures for spontaneous differentiation and contamination. - bachelors degree in the biological sciences, including molecular biology, biochemistry or a related discipline - 1-2 years of laboratory experience which could include classroom laboratory experience or employment - Hands-on experience with sterile mammalian cell culture, including independent day-to-day maintenance of continuous cultures - Preference will be given to applicants who seek at least 2 years of commitment to this position. We could combine the associate requirements as alternative but we still list them. Now Skills (desired qualifications): - Two or more years of dedicated human pluripotent stem cell culture experience in an academic or industry laboratory - Experience with directed differentiation protocols and with iPSC-derived neuronal, organoid cultures - Experience with live-cell and confocal imaging and with flow cytometry cultured cells - Experience supporting plate-based or high throughput screening workflows, including 24-96-well culture, liquid handling, and assay quality control - Familiarity with basic techniques in biomedical research, DNA/RNA extraction and purification, quantitative real-time PCR, Western blotting, and fluorescence microscopy - Experience using CRISPR techniques in cell culture, particularly gene editing of iPSC lines, single-cell cloning, and screening and validation of edited clones - Experience with tissue processing and immunohistochemistry in fixed tissues Now Schedule: - Participation in a rotating weekend and holiday feeding schedule shared across the lab. We could phrase as
Participation in a rotating weekend and holiday feeding schedule shared across the lab.
- for lists.
We need to ensure we preserve all substantive information. Let's craft.
About the role: we need to include the whole job summary paragraph as prose. Might be long but okay.
We'll wrap in