Research Associate (Single Cell Transcriptomics)

Location: South San Francisco, CA, 94080

Responsibilities

• Design, plan and execute experiments using single cell RNA sequencing pipeline from sample preparation and capture to NGS sequencing library preparation and QC with high precision
• Prepare single cell and single nuclei samples for next-generation sequencing (NGS) and other applications
• Conduct fluorescence and/or magnetic-activated cell sorting experiments
• Help optimize and improve workflow, and streamline processes
• Collect, organize, and manage sample inventory
• Organize and maintain detailed experimental records in accordance with industry standards
• Manage reagent inventory and equipment calibration/maintenance
• Collaborate with multidisciplinary teams and colleagues
• Analyze data, interpret and present results at team meetings
• Support other Discovery projects involving human neuronal cell culture and cell-based assays

Requirements

• BS or MS degree in Biology, Molecular/Cell Biology or related field
• Experience with single cell NGS sample prep workflow (cell capture using microfluidics, cDNA and library preparation, QC and sequencing)
• Experience with flow cytometry and fluorescence activated and/or magnetic cell sorting (FACS/MACS)
• Familiarity with DNA/RNA library QC techniques (Tapestation, Bioanalyzer, Qubit, etc.)
• Working knowledge of standard molecular biology techniques (PCR, qPCR, gel electrophoresis, DNA/RNA extraction)
• Strong organizational and analytical skills
• Willingness to perform repetitive tasks with high accuracy and superb attention to detail
• Willingness to learn and expand skill set and contribute to emerging needs in a highly dynamic start-up environment
• Ability to multitask while working within fast-paced timelines
• High integrity, excellent written, verbal and interpersonal communication skills
• Strong team player and problem-solver, able to work on teams and independently

Preferred Qualifications

• 2+ years of relevant hands-on laboratory experience in academia or industry
• Familiarity with single-cell RNA sequencing data analysis
• Familiarity with histochemical techniques and basic microscopy
• Experience with sterile human stem cell and neuronal cell culture
• Basic knowledge of stem cell biology and neurodevelopmental biology