Research Associate I

About the role

The mission of the Allen Institute is to unlock the complexities of bioscience and advance our knowledge to improve human health. Our lab focuses on applying machine learning to biological design, building a series of interconnected design-test-loop "flywheels" that enable the design of synthetic enhancers, protein binders, and more.

Responsibilities

• Maintain and propagate human cell lines
• Optimize and perform plasmid DNA transfections and viral transductions
• Execute molecular biology protocols, including molecular cloning, flow cytometry, quantitative PCR, ddPCR, Illumina amplicon sequencing, RNA-seq, single cell RNA-seq
• Assist in troubleshooting technical barriers in the generation of high quality NGS libraries
• Contribute to the development of new protocols as needed
• Present results and summarize progress to help frame internal decision-making
• Comply with SOPs, good laboratory practices, and regulatory requirements
• Maintain accurate laboratory documentation using established methods
• Other duties as assigned

Requirements

• Bachelor’s degree in Cell Biology, Genetics, Synthetic Biology, Bioengineering, Genomics, Molecular Biology, Bioinformatics, or related field
• 2 years of equivalent experience
• Experience with stem cell culture
• Experience performing molecular biology methods including molecular cloning (such as restriction cloning, Gibson Assembly, Golden Gate assembly, plasmid prep)
• Experience cloning complex libraries is a plus
• RNA isolation, DNA isolation, and PCR track-record
• Experience performing cell biology methods including mammalian cell culture, stem cell culture, primary cell culture, nucleofection, and lipofection

Qualifications

• Ability to work independently and as part of a team
• Strong attention to detail and organizational skills
• Excellent communication and presentation skills
• Ability to work flexible hours and travel as needed

Skills

• Experience with nucleic acid sequencing, especially custom amplicon sequencing
• Experience with laboratory animal handling

Benefits

Annualized Salary Range: $54,250 - $66,450
Final salary depends on required education for the role, experience, and level of skills relevant to the role, along with work location, where applicable.
Details on the Allen Institute’s benefits offering are located at the following link to the Benefits Guide: https://alleninstitute.org/careers/benefits

Pay

Annualized Salary Range: $54,250 - $66,450

Schedule

This role requires onsite work and is expected to work onsite for the majority of the working hours. We are a Washington State employer, and the primary work location for Allen Institute employees is 700 Dexter Ave N.; any remote work must be performed in Washington State.

Additional Comments

Please note, this opportunity may offer relocation assistance. Please note, this opportunity does not provide work visa sponsorship.