Research Associate-Burclaff Lab

About the role

The Burclaff Lab focuses on understanding how metabolism and cellular energetics are regulated within intestinal stem cells, and how this dynamic regulation supports homeostasis, identity maintenance, fate decisions, cell cycle regulation, and tissue plasticity following injury or disease. We utilize a mix of animal models and cutting-edge, genetically modified, primary human intestinal stem cell cultures to advance our understanding of the mammalian intestine.

Responsibilities

• Establish, propagate, and maintain mammalian primary cell cultures and established cell lines using aseptic techniques to support research projects.
• Perform routine and advanced molecular biology and biochemical assays, including DNA/RNA/protein extraction, PCR, RT-qPCR, gel electrophoresis, tissue sectioning, immunofluorescence, immunohistochemistry staining, and live image analysis.
• Oversee mouse colony operations—including handling, breeding, weaning, genotyping, and tissue collection—and execute experimental procedures in accordance with institutional guidelines.
• Maintain, calibrate, and troubleshoot laboratory equipment, manage inventory and data logs, and ensure strict compliance with safety protocols and standard operating procedures.
• Organize and maintain research data, draft or edit documents, and contribute to publications, grant applications, and presentations.

Requirements

• Relevant post-Baccalaureate degree, or relevant Bachelor’s degree plus 3 or more years of independent research experience.
• Experience in mammalian primary cell culture (establishment and maintenance).
• Experience in molecular biology and biochemistry techniques (PCR; RT-qPCR; gel electrophoresis, DNA, RNA, and protein extraction; immunofluorescence; immunohistochemistry).
• Experience in mouse colony management (animal handling, breeding, weaning, genotyping, etc.) and in vivo experimentation.

Preferred Qualifications

• Prior research experience.
• Ability to work with animals.
• Strong computer skills and ability to use highly specialized laboratory equipment with troubleshooting abilities.

Benefits

• Medical, Dental, and Vision
• Flexible Spending Account
• Retirement Programs
• Disability Plans
• Life Insurance
• Accident Plan
• Paid Time Off and Other Leave Programs
• 12 Holidays Each Year
• Tuition and Academic Assistance
• Childcare benefits
• Wellness & Recreation Membership
• Wellness Programs