Research Assistant III/IV - Oncology (AML Research Lab)

Job Responsibilities

• Perform and troubleshoot molecular and cellular biology experiments with minimal supervision.
• Maintain and characterize leukemia cell lines and primary patient samples.
• Conduct tissue culture studies, in vitro drug treatment experiments, and mechanistic studies of therapeutic response and resistance.
• Perform protein-based analyses, including immunoblotting, immunoprecipitation (IP), coimmunoprecipitation (co-IP), and related assays.
• Isolate DNA and RNA from cells, tissues, and animal samples.
• Prepare samples for next-generation sequencing and assist with sequencing-based studies.
• Support in vivo studies, including planning, coordination, sample collection, and analysis of mouse experiments.
• Maintain accurate, detailed, and well-organized experimental records and laboratory notebooks.
• Analyze, summarize, and present experimental findings at laboratory meetings.

Job Qualifications

• Required for RAIII: Bachelor's degree in a related field AND 1 year of directly related experience OR Master's degree in a related field
• Required For RAIV: Bachelor's degree in a related field 3 years of experience in related job discipline OR Master's degree and 2 years experience
• Preferred Qualifications: Demonstrated experience with: Mammalian tissue culture, DNA and RNA isolation, Protein biochemistry techniques, including immunoblotting and immunoprecipitation, Molecular biology techniques and sequencing workflows, In vitro drug treatment studies, Mouse research and in vivo experimental planning, Strong organizational, record-keeping, and project management skills, Ability to work both independently and collaboratively in a fast-paced research environment, Experience in leukemia, hematologic malignancies, cancer biology, or drug resistance research, Experience handling primary patient-derived samples, Familiarity with next-generation sequencing approaches and downstream data interpretation, Previous laboratory management or supervisory experience, Experience working in NIH-funded academic research environments.

About Us

Cincinnati Children’s, recognized by U.S. News & World Report as a top 10 best Children's Hospitals in the nation for more than 15 years, consistently among the top 3 Children's Hospitals for National Institutes of Health (NIH) Funding. We are a Leading Disability Employer as noted by the National Organization on Disability, Magnet® designated for the fourth consecutive time by the American Nurses Credentialing Center (ANCC), and a Magnet® designated hospital. We are proud to be an Equal Opportunity Employer committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status. EEO/Veteran/Disability