Research Assistant II Lab
Harvard Medical School · Boston, MA · 1 wk ago
On-siteAnalystFull-time
About the role
The Lu Lab at Harvard Medical School seeks a Research Assistant to support small-molecule discovery targeting the ubiquitin–proteasome system (UPS).
Responsibilities
- Execute and optimize biochemical and cell-based assays to evaluate small-molecule inhibitors.
- Support hit validation and SAR workflows in collaboration with external partners.
- Analyze experimental data and maintain accurate, reproducible records.
- Maintain mammalian cell cultures, including maintenance of engineered cell lines.
- Perform molecular cloning, including plasmid design, PCR, lentiviral production, and stable cell line generation.
- Conduct western blotting, protein expression analysis, and microscopy-based assays.
- Express and purify recombinant proteins from both E. coli and mammalian cells.
- Assist in biochemical characterization of enzyme activity and inhibitor interactions.
- Support mouse work, including colony maintenance and experimental procedures.
- Assist with lab management tasks such as ordering, inventory, equipment maintenance, and safety trainings.
Qualifications
- College background in Biology, Biochemistry, Chemical Biology, or a related field, with 1-2 years of relevant laboratory experience.
- Experience in protein biochemistry, including protein purification, enzymatic assays, western blotting.
- Familiarity with mammalian cell culture and genetic manipulation (CRISPR, shRNA, or lentiviral systems).
- Experience in molecular cloning (PCR, restriction digest, Gibson Assembly, Goldengate Assembly, transformation, etc.).
- Basic data analysis/programming skills.
- Exposure to drug screening or chemical biology approaches is highly desirable.
- Experience with mouse handling is a plus (not required).