Research Assistant - 140190

About the role

This position provides support in a research setting, assisting with various molecular and cellular biology experiments, animal studies, and data analysis.

Responsibilities

• Assist with experiments, making observations, and recording data.
• Use molecular biological techniques to develop plasmid and viral-based gene transfer vectors.
• Assist with animal studies, including breeding, injections, and maintaining transgenic mouse colonies.
• Perform in vivo imaging, necropsy, tissue trimming, and histology.
• Prepare tissue cultures and propagate tumor cell lines.
• Extract RNA, reverse transcribe cDNA, perform RT-PCR, and analyze plasmids by gel electrophoresis.
• Conduct basic cellular immunology tests such as FACS and CFSE proliferation assays, and perform cell counting.
• Prepare media and buffers as needed.
• Maintain lab records and order supplies as necessary.

Requirements

• Theoretical knowledge of biochemistry, molecular biology, immunology, and genetics, including relevant laboratory techniques, obtained through a Bachelor’s degree in biology or related field.
• Experience with tissue culture, including freezing, thawing, counting cells, and splitting cultures.
• Experience with data analysis using Excel and Prism software.
• Experience with aseptic techniques and tissue culture.
• Ability to work with human tissues, including tissue trimming, histology, and necropsy.
• Knowledge of cell culture, media preparation, and DNA electrophoresis.
• Willingness to follow step-by-step experimental protocols.
• Ability to use basic laboratory equipment, including centrifuges, spectrophotometers, balances, pH meters, and electrophoresis equipment.
• Willingness to handle chemical and biohazard materials safely.
• Willingness to adhere to UCSD safety guidelines.
• Familiarity with computer software for data analysis and storage.
• Willingness to maintain confidentiality and organize lab equipment and supplies.
• Ability to perform basic PCR and ELISA techniques.
• Ability to work with a variety of chemicals and biological materials while adhering to safety procedures.
• Willingness to work various hours and locations as needed.

Preferred Qualifications

• Experience with patient databases and EPIC.
• Experience with small lab animals (mice), including administering medications, anesthesia, and performing phlebotomy and minor surgical procedures.
• Knowledge of hematopoietic stem and progenitor cell biology, microbiology, molecular biology, and prostate cancer biology.
• Understanding of prostate cancer disease models and clinical features.

Special Conditions

• Must be able to work various hours and locations based on business needs.
• Employment is subject to a criminal background check.

Pay

Annual Full Pay Range: $59,905 - $71,451 (prorated if appointment percentage is less than 100%)

Benefits

Not specified in the job posting.

Schedule

Not specified in the job posting.