Postdoctoral Researcher

Postdoctoral Research Fellow- Wang LaboratoryDivision of Pulmonary MedicineBoston Children's Hospital | Harvard Medical School Description: The Wang Laboratory at Boston Children's Hospital and Harvard Medical School is seeking a motivated postdoctoral fellow to study airway epithelial biology using human induced pluripotent stem cell (iPSC) models and human primary airway models. Our laboratory is interested in how specialized airway epithelial cells develop and function in health and disease. A major focus is pulmonary ionocytes, a rare epithelial cell population enriched for CFTR expression. We use human iPSC-derived airway models together with CRISPR genome editing, single-cell sequencing, and functional assays to investigate airway development, epithelial homeostasis, pediatric lung diseases, and respiratory infections. The successful candidate will have the opportunity to lead an independent research project while working in a collaborative environment with investigators across Boston Children's Hospital and Harvard Medical School. Research AreasProjects in the laboratory includeDevelopmental pathways regulating pulmonary ionocyte differentiationIdentification of pathways and compounds that regulate ionocyte differentiationCell-cell interactions within the airway epitheliumHuman iPSC models of lung diseaseMechanisms of epithelial ion transport and CFTR functionAirway responses to influenza, RSV, coronavirus, and Pseudomonas aeruginosa, working with multiple collaborators on Harvard campus and throughout the greater Boston area and UnitSingle-cell analysis of airway epithelial remodeling Experimental ApproachesDirected human iPSC differentiationAir-liquid interface and airway organoid culturesCRISPR/Cas9 genome editingInducible gene expression and knockdown systemsFluorescent reporter cell linesImmunofluorescence and confocal microscopySingle-cell RNA sequencingAirway electrophysiology (Ussing chamber)Functional assays of epithelial ion transportViral and bacterial infection models ResponsibilitiesDesign and perform independent research studiesAnalyze and interpret experimental dataPrepare manuscripts and conference presentationsContribute to grant applicationsMentor students and research staffParticipate in collaborations within Boston Children's Hospital and Harvard Medical School The LaboratoryThe laboratory is part of the Division of Pulmonary Medicine at Boston Children's Hospital and is supported by NIH and foundation funding. Fellows have access to outstanding institutional resources, including genomics, imaging, gene editing, bioinformatics, and stem cell core facilities.We encourage fellows to develop independent research directions and provide mentorship in manuscript preparation, grant writing, and career development. QualificationsRequiredPhD, MD/PhD, or equivalent in cell biology, developmental biology, stem cell biology, pulmonary biology, immunology, molecular biology, bioengineering, or a related fieldStrong written and verbal communication skillsAbility to work independently and as part of a collaborative research teamPreferredExperience with one or more of the following is desirable but not required:Human iPSC cultureCRISPR genome engineeringOrganoid or airway epithelial cultureSingle-cell genomicsMicroscopyHost-pathogen interactionsComputational analysis . ApplicationInterested applicants should submitCover letterCurriculum vitaeContact information for three references toRuobing (Ruby) Wang, MDAssistant Professor of PediatricsDivision of Pulmonary MedicineBoston Children's HospitalHarvard Medical SchoolEmail: Ruobing.Wang@childrens.harvard.edu Pay TransparencyExempt Role: 70,000-77,267, depending on PGY level and experienceThe posted pay range is Boston Children’s reasonable and good-faith expectation for this pay at the time of posting. Pay offer provided depends on skills, experience, education, certifications, and a variety of other job-related factors. BCH offers a comprehensive benefits package that includes flexible schedules, medical/vision/dental coverage, child-care and student-loan subsidies, generous paid time off, a 403(b) retirement plan, commuter and other employee discounts. Boston Children’s Hospital requires influenza vaccination for all employees, unless eligible for a medical or religious exemption. Boston Children’s Hospital is an Equal Opportunity/Affirmative Action Employer; all qualified applicants will receive consideration without regard to race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status, disability, or other protected status.