Laboratory Manager II

Med-Microbiology and Molecular Genetics – The Kamil Lab in the University of Pittsburgh School of Medicine, Department of Microbiology and Molecular Genetics is recruiting an experienced Laboratory Manager II. The Kamil laboratory studies diverse aspects of the molecular interplay between viruses and their hosts, focusing primarily on human cytomegalovirus (aka HCMV or CMV). The laboratory is investigating how CMV exploits glycobiology to hide within the host and are also studying a new virion surface glycoprotein complex that plays roles in entry, which we recently discovered together with our collaborators. The lab is also interested in the fundamental cell biology underlying how CMV overtakes the host cell and manipulates the secretory pathway.

Responsibilities

• Manage day-to-day laboratory operations while ensuring compliance with safety regulations and best practices.
• Work with, adapt, and install scientific equipment in research laboratories.
• Develop and collaborate on routine projects and procedures.
• Manage inventory and equipment and perform budget functions.
• Work closely with the faculty investigator to align day-to-day laboratory operations with long-term research goals while keeping near-term projects on schedule.
• Maintain inventory of reagents and supplies and handle routine purchasing of materials and equipment.
• Independently design and execute experiments with minimal oversight, including:
  • Propagation, purification, and titration of virus stocks (plaque assay and/or TCID50).
  • Design and execution of infection time-course experiments.
  • Mammalian cell culture and aseptic technique, including primary fibroblasts and epithelial cells.
  • Cryopreservation and maintenance of cell banks.
  • Standard molecular biology techniques: PCR, quantitative PCR (qPCR and RT-qPCR), isolation of DNA and RNA, gel electrophoresis, recombinant DNA and subcloning, site-directed mutagenesis, genetic techniques in bacteria.
  • Generation of recombinant viruses and/or engineered cell lines (highly desirable).
• Interpret data, develop and troubleshoot new methods and procedures.
• Maintain excellent record keeping and communication skills.

Requirements

• Extensive hands-on laboratory experience working with viruses at Biosafety Level 2.
• Bachelor’s Degree in a relevant field.
• Minimum of 3 years of relevant experience (combination of education and relevant experience will be considered in lieu of education and/or experience requirement).

Physical Effort

The incumbent will spend most of the day moving about the laboratory and will be required to lift light boxes and equipment.

Schedule

Monday–Friday, 8:00 AM–4:30 PM.

Work Arrangement

On-Campus: Teams that work on campus, in an office, or in a lab.