Junior/Assistant/Associate/Full Specialist (Neuroscience and Protein Engineering)
University of California, San Francisco · San Francisco, CA · 3 mo ago
Engineering$55k–$195k/yrFull-time
Primary Responsibilities
- Cloning recombinant protein and antibody constructs using a variety of molecular biology techniques including DNA purification, transformation, polymerase chain reaction, and Gibson assembly.
- Expressing proteins from E.coli and mammalian sources (HEK293-F and CHO cells).
- Purifying proteins using chromatography techniques including Protein A, immobilized metal affinity chromatography, and ion exchange chromatography.
- Qualifications
- Junior Specialist: Baccalaureate degree in biochemistry, cell biology, or relevant life sciences field or at least four years of research experience.
- Assistant Specialist: Master’s degree in biochemistry, cell biology, or relevant life sciences field, OR Baccalaureate degree in biochemistry, cell biology, or relevant life sciences field with three or more years of research experience.
- Associate Specialist: Master’s degree in (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or five to ten years of research experience.
- Full Specialist: Terminal degree (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or ten or more years of research experience.
Additional Qualifications
- Junior Specialist: At least one year of wet laboratory experience.
Preferred Qualifications
- Expertise in protein handling, expression, and purification.
- Proficiency with mammalian tissue culture techniques including transfections, cell line maintenance, and recombinant protein expression.
- Familiarity with immunology techniques such as immune cell isolation, culturing, expansion, and cytotoxicity assays.
- Proficiency with advanced biophysical techniques such as Biolayer Interferometry (BLI), Surface Plasmon Resonance (SPR), poly-reactivity assays, and HPLC chromatography.
- Data analysis experience using programming languages such as R or Python.