Histotechnologist
About the role
We are looking for a Histotechnologist to own and execute histology workflows at Nudge, focusing primarily on mouse and rat brain tissue. You will be responsible for building the full histology pipeline, including perfusion, prosection, fixation, embedding (paraffin and OCT), sectioning via microtome, cryostat, and vibratome, standard histological stains (H&E, special stains), and immunohistochemistry using both manual and automated staining platforms. You will also operate and optimize tissue clearing platforms (electrophoretic, solvent-based) for whole-brain and thick-section imaging, prepare cleared tissue for light sheet fluorescence microscopy and volumetric 3D imaging, operate and maintain automated sectioning and tissue processing systems, prepare and process samples for spatial omics platforms, including multiplexed protein imaging and spatial transcriptomics, with both RNA- and protein-based tagging approaches, and develop, optimize, and document SOPs for histological workflows, ensuring reproducibility and quality. You will collaborate with research scientists to adapt tissue processing and staining strategies to specific experimental questions.
Requirements
- At least 3 years of industry experience in histotechnology, with significant time spent processing rodent brain tissue
- Hands-on experience in histotechnology, proficiency across core histological techniques: microtomy (paraffin, cryo, vibratome), tissue embedding, H&E staining, and immunohistochemistry
- Experience with at least one tissue clearing method and associated imaging modalities (light sheet microscopy)
- Familiarity with automated staining and sectioning platforms
- HT(ASCP) certification or equivalent training
- Bachelor’s degree in biology, neuroscience, or a related field preferred
Qualifications
- High integrity and strong professional judgment