Flow Cytometry Technician I

About the role

Operate and maintain a diverse suite of analytical instruments (including BD Symphony A5, BD LSR Fortessa, Bio-Rad ZE5, and Beckman Coulter Cytoflex) to ensure peak performance and user readiness. Independently operate, calibrate, and provide expert cell sorting services using advanced cell sorters (e.g., BD S6, Bio-Sorter). Perform daily QC, preventative maintenance, and tracking for all core instrumentation; maintain detailed, accurate logs and documentation. Execute first-level instrument troubleshooting to resolve common hardware and software issues. Escalate complex instrument failures to the Core Leader and coordinate service visits with external vendors. Ensure strict adherence to Core Standard Operating Procedures (SOPs) and institutional safety protocols, including EH&S and Biosafety Level 2 (BSL2+) regulatory requirements.

Responsibilities

• Operate and maintain a diverse suite of analytical instruments (including BD Symphony A5, BD LSR Fortessa, Bio-Rad ZE5, and Beckman Coulter Cytoflex)
• Independently operate, calibrate, and provide expert cell sorting services using advanced cell sorters (e.g., BD S6, Bio-Sorter)
• Perform daily QC, preventative maintenance, and tracking for all core instrumentation; maintain detailed, accurate logs and documentation
• Execute first-level instrument troubleshooting to resolve common hardware and software issues. Escalate complex instrument failures to the Core Leader and coordinate service visits with external vendors
• Ensure strict adherence to Core Standard Operating Procedures (SOPs) and institutional safety protocols, including EH&S and Biosafety Level 2 (BSL2+) regulatory requirements

Requirements

Requires some physical activity, including equipment setup, reagent handling, and light lifting (up to 50 lbs.).

Preferred Qualifications

• Education: Bachelor’s degree in Biology, Immunology, Molecular Biology, Biochemistry, or a related scientific field
• Flow Cytometry Expertise: Significant hands-on experience in multi-color flow cytometry, including sample preparation, data acquisition, and data analysis using software such as FlowJo, FCS Express etc.
• Cell Sorting Experience: Demonstrated proficiency in the independent operation, calibration, and maintenance of cell sorters to provide reliable cell sorting services
• Technical Literacy: Familiarity with standard computer systems, literature search methodologies, and data plotting/statistical analysis
• Prior experience in spectral flow cytometry and imaging technologies is highly preferred
• Excellent verbal and written communication, organizational, and record-keeping skills
• Proven ability to work independently, adaptable team player committed to supporting a collaborative research environment

Education And Experience

• Required Education and Experience: Bachelor’s Degree in an Engineering or Scientific discipline
• Experience in a laboratory/research setting
• Demonstrated knowledge in solving technical problems such as issues with lab/flow cytometry instrumentation

Preferred Experience

• Acquiring and analyzing Flow Cytometry data, preferably with BD FacsDiva software
• Data entry and/or word processing experience
• Experience in analyzing data using spreadsheets and statistical analysis

Compensation Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

Benefits Information

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits.

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE