Asst Research Scientist/Engin - Li Lab

About the role

The Department of Pathology at UI Health Care is seeking an Assistant Research Scientist to investigate the mechanisms of how A-FABP and E-FABP regulate cell metabolism and intracellular signaling pathways in cells from various disease models such as obesity, cancer, and psoriasis.

Responsibilities

• Design and perform assigned research projects to obtain high-quality data in each individual study.
• Manage laboratory protocols, train and collaborate with laboratory personnel, including students, post-doctoral fellows, and technicians.
• Cell culture for cell lines, primary immune cells like BMDC, BMDM, establish cell lines of stable gene expression or knockout using CRISPR technique.
• Preparation of single cell suspension from tissues.
• Highly skilled in real-time PCR, western blotting, RNAi; perform antibody generation, isolation, and purification.
• Perform ELISA from serum and supernatant.
• Perform Immunofluorescence staining using cells and tissues.
• Maintenance of mouse colonies and genotyping with assistance of technician.
• Prepare, perform, and analyze samples using multi-color flow cytometry.
• Troubleshoot experimental procedures and update as needed.

Requirements

• Ph.D. in Immunology
• At least 1 year of postdoctoral experience in Immunology or cancer-related field
• Strong ability to independently design, optimize, and perform experiments, establish experimental protocols to solve scientific questions
• Evidence of publications in peer-reviewed journals
• Establishing a national presence with talks, abstract presentations, and/or participation at professional meetings
• Excellent communication skills and positive team effort

Desirable Qualifications

• At least 3 years of postdoctoral experience in Immunology or cancer-related field
• Prior experience with developing mouse models on high fat diet/tumor implantation, and compliance with IACUC regulations
• Highly skilled in multi-color Flow cytometry to identify immune cells (T cell, macrophages)
• Standard molecular biological and immunological techniques